dq™ collagen type iv Search Results


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MedChemExpress col1
Biological functional analysis of tri-layer scaffold systems. A) Representative images of migrated cells stained with crystal violet at 48 h. B) Number of migrating cells in control and CE-SKP. C) Glycosaminoglycan concentrations in control and CE-SKP at 7 and 14 days. D) qPCR analysis for COL2, SOX9, and ACAN. E) Western blotting of COL2 and SOX9. F) Alizarin red staining of control and CPH. G) ALP activity of control and CPH. H) qPCR analysis for <t>COL1,</t> COL10, OCN, and RUNX2. I) Western blotting of COL10, COL1, and RUNX2. J) Representative images of migrated cells stained with crystal violet at 48 h. K) Number of migrating cells in control and P2G3. L) Scanning electron microscope micrographs of P2G3. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
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Proteintech col4a2 polyclonal antibody
A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and <t>COL4A2</t> gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.
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SouthernBiotech collagen iv
A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and <t>COL4A2</t> gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.
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Rockland Immunochemicals anti type iv collagen
A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and <t>COL4A2</t> gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.
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SouthernBiotech goat polyclonal
A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and <t>COL4A2</t> gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.
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Aviva Systems recombinant type iv α5 collagen
A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and <t>COL4A2</t> gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.
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SouthernBiotech jama network open
A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and <t>COL4A2</t> gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.
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Biosynth Carbosynth anti collagen iv
A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and <t>COL4A2</t> gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.
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SouthernBiotech human collagen type iv
A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and <t>COL4A2</t> gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.
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Boster Bio collagen iv
A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and <t>COL4A2</t> gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.
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ProSci Incorporated cert
A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and <t>COL4A2</t> gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.
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Image Search Results


Biological functional analysis of tri-layer scaffold systems. A) Representative images of migrated cells stained with crystal violet at 48 h. B) Number of migrating cells in control and CE-SKP. C) Glycosaminoglycan concentrations in control and CE-SKP at 7 and 14 days. D) qPCR analysis for COL2, SOX9, and ACAN. E) Western blotting of COL2 and SOX9. F) Alizarin red staining of control and CPH. G) ALP activity of control and CPH. H) qPCR analysis for COL1, COL10, OCN, and RUNX2. I) Western blotting of COL10, COL1, and RUNX2. J) Representative images of migrated cells stained with crystal violet at 48 h. K) Number of migrating cells in control and P2G3. L) Scanning electron microscope micrographs of P2G3. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Journal: Bioactive Materials

Article Title: Bioinspired scaffold recapitulating chondrogenic ontogeny and microenvironment for functional cartilage regeneration

doi: 10.1016/j.bioactmat.2025.11.041

Figure Lengend Snippet: Biological functional analysis of tri-layer scaffold systems. A) Representative images of migrated cells stained with crystal violet at 48 h. B) Number of migrating cells in control and CE-SKP. C) Glycosaminoglycan concentrations in control and CE-SKP at 7 and 14 days. D) qPCR analysis for COL2, SOX9, and ACAN. E) Western blotting of COL2 and SOX9. F) Alizarin red staining of control and CPH. G) ALP activity of control and CPH. H) qPCR analysis for COL1, COL10, OCN, and RUNX2. I) Western blotting of COL10, COL1, and RUNX2. J) Representative images of migrated cells stained with crystal violet at 48 h. K) Number of migrating cells in control and P2G3. L) Scanning electron microscope micrographs of P2G3. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: To evaluate the regeneration of cartilage, COL1 (1:100, HY- P81227 , MCE), COL2 (1:100, ARG20787 , Arigobio), and COL10 (1:100, BA2023, Boster) were used as a marker in immunohistochemistry (IHC) staining.

Techniques: Functional Assay, Staining, Control, Western Blot, Activity Assay, Microscopy

Regeneration of the femoral trochlea in a rabbit model. Representative images of IHC staining for A) COL1, B) COL10.

Journal: Bioactive Materials

Article Title: Bioinspired scaffold recapitulating chondrogenic ontogeny and microenvironment for functional cartilage regeneration

doi: 10.1016/j.bioactmat.2025.11.041

Figure Lengend Snippet: Regeneration of the femoral trochlea in a rabbit model. Representative images of IHC staining for A) COL1, B) COL10.

Article Snippet: To evaluate the regeneration of cartilage, COL1 (1:100, HY- P81227 , MCE), COL2 (1:100, ARG20787 , Arigobio), and COL10 (1:100, BA2023, Boster) were used as a marker in immunohistochemistry (IHC) staining.

Techniques: Immunohistochemistry

A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and COL4A2 gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.

Journal: NPJ Science of Food

Article Title: Haematococcus pluvialis ameliorates renal fibrosis by restoring mitophagy via PINK1-Parkin-p62-LC3 signaling

doi: 10.1038/s41538-025-00654-x

Figure Lengend Snippet: A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and COL4A2 gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.

Article Snippet: LC3 Polyclonal antibody (14600-1-AP), p62 Polyclonal antibody (80294-1-RR), PINK1 Polyclonal antibody (23274-1-AP), Parkin Polyclonal antibody (14060-1-AP), E-cadherin Polyclonal antibody (20874-1-AP), N-cadherin Polyclonal antibody (No.: 22018-1-AP), Vimentin Polyclonal antibody (10366-1-AP), COL4A2 Polyclonal antibody (55131-1-AP), FN1 Monoclonal antibody (66042-1-Ig), β-Actin Polyclonal antibody (20536-1-AP), and α-Tubulin Polyclonal antibody (11224-AP) were from Proteintech group Co., Ltd (Wuhan, China).

Techniques: Staining, Expressing, Immunohistochemistry, Western Blot, Control, Functional Assay, RNA Sequencing